Reports of damaged vegetation in the road reserve along the Stawell-Donald Road sparked concern from residents, with authorities called in to investigate.
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The damage occurred on both sides of Stawell-Donald Road, around 12km from Stawell.
The stretch of damaged road reserve is notable, with some scorch marks on tree trunks and many leaves dried, almost to a crisp, while other leaves on the same trees remain healthy.
Some residents believed the damage might have been caused by farming chemicals that drifted through the air when sprayed by an aeroplane.
Leading to officers from the Department of Environment, Land, Water and Planning, Agriculture Victoria and the Northern Grampians Shire Council investigating the scene.
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However, officers from multiple authorities have confirmed the misuse of chemicals did not cause the damage.
A spokesperson from Agriculture Victoria said: "an Agriculture Victoria officer attended the site with the Northern Grampians Shire Council and the Department of Environment, Land, Water and Planning on May 19, it was determined the damage was not chemical related."
"For more information, Agriculture Victoria advises contacting the local council."
Northern Grampians Shire Council mayor Murray Emerson said the CFA was also notified of the issue.
"Officers from Northern Grampians Shire Council, DELWP and Agriculture Victoria attended the site to inspect the reported damage on May 19," he said.
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"Our inspection determined that the most likely cause of the vegetation damage would have been from a stubble burn."
"CFA have also been notified."
A CFA spokesperson confirmed the Stawell brigade has been notified of the issue and said the strong heat of stubble burns could radiate longer distances and impact vegetation without flames leaving the property.
In Victoria, a permit is usually required to remove, destroy or lop native vegetation. These regulations are known as the native vegetation removal regulations and are primarily implemented through local council planning schemes.
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