Update Friday 11.30am: Coaches will replace trains between Wendouree and Ararat on Friday, as construction continues on the Ballarat Line upgrade.
V-Line has advised Ararat customers to leave an extra 45 minutes for their journey.
A V-Line spokesman said crews were working on a new section of duplicated track and a second platform at Wendouree, near Ballarat.
"Once completed, the duplicated track will improve reliability for Ararat and Wendouree services by providing an additional place for trains to cross," he said.
Thursday: V/LINE commuters in the Ararat, Ballarat and Maryborough lines have been left waiting for the bus due to industrial action Thursday.
All trains on Thursday on the Ararat, Ballarat and Maryborough line were cancelled and replaced by buses in the second day of a ten-day industrial campaign by public transport union group the Rail, Train, and Bus Union.
Coaches will replace trains on the Ararat and Maryborough lines for the entire journey for the remainder of the day.
Due to industrial action, coaches will replace trains all day today on the Ballarat line. Freight trains and other services will still operate so always obey warning signs at level crossings and stay safe around trains.— V/Line Ballarat Line (@vline_ballarat) December 11, 2019
For more information, please visit https://t.co/QjMlJmvRTypic.twitter.com/PBqQo3Pnca
On its website Public Transport Victoria advised passengers arrive in time for services as scheduled on the timetable and allow an extra 45 minutes for their journeys.
All train services have been replaced by a mix of express and stopping all stations coach services.
The Public Transport Victoria website, app, social media channels and station displays include updated travel information.
Member for Ripon Louise Staley said the action reflected the state government's "mismanagement" of regional rail.
"Once again, passengers in Ripon have borne the brunt of Labor's mismanagement of Victoria's regional passenger rail," Ms Staley said.
"Daniel Andrews and Labor have arrogantly ignored regional rail commuters for too long. This is yet another example of their attitude towards those Victorians who don't live on the tram tracks.
"People in Ripon need reliable and safe public transport to go about their daily lives, but Labor does not care about this. It is high time Daniel Andrews and Labor listened to regional Victorians and ensures this vital service operates as it is needed."