Overnight, three people were arrested for earlier obstructing police at the site of the protest charged with offences including intentionally obstructing an emergency service worker on duty, refusing to leave a restricted access area and failing to comply with the Chief Health Officers directions.

All three people were bailed to reappear at Ararat Magistrates Court at a later date.

There were two protesters remaining in a tree on Wednesday morning, one since being arrested and taken to the Ararat Police Station.

Victoria's Supreme Court ordered works to stop on the Western Highway duplication on Tuesday evening.

The latest arrests are a result of a police operation where more than 50 people were arrested as police removed protesters and camps along the Western Highway duplication route.

READ HOW TUESDAY UNFOLDED HERE

Victoria Police had a "dedicated" presence at the Western Highway protest camps on Monday.

More to come.

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