Thousands of protesters demanding the resignation of President Jovenel Moise have clashed with police as some tried to storm barriers outside the National Palace.
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The demonstration came a day after Moise rejected demands that he step down over allegations of officials misusing funds from subsidised oil shipments from Venezuela under the Petrocaribe program.
He denied any wrongdoing.
The latest protest followed a strike Monday and Tuesday that shuttered schools, businesses and government offices, shut down public transportation and caused a Parliament session to be suspended.
Burning tyres still blocked some streets and it was difficult to reach some towns outside Port-au-Prince.
A Senate investigation has said at least 14 former government officials allegedly misused $3.8 billion under the administration of former President Michel Martelly. A report that was recently released levelled new accusations against government officials.
A second protest has been planned despite President Moise calling for people to stay calm in a brief speech as he celebrated the anniversary of Haiti's police force.
"I am not going to allow anyone to commit chaos under any pretext," the president said. "I am asking you to stay calm."
Australian Associated Press