The Grampians was the most recent destination ticked off by eco-tourism organisation Trash Bags on Tour.
The company visited the Grampians on Sunday, taking the group of 12 people around the national park picking up rubbish for 50 minutes before seeing the sites including Lake Bellfield and MacKenzie Falls.
"It was a really awesome experience in and around Halls Gap and the Grampians," tour co-director Kathryn Farrell said.
The group picked up 26 kilograms of litter as part of the tour.
"It is a little above average from the other tours we do," Ms Farrell said.
"One interesting thing we noticed was we picked up about 30 plastic ice cream spoons which is quite a lot in less than an hour of work."
Ms Farrell said she has intentions of bringing her company back to the national park in the future for more tours of the like.
"Everyone we brought along loved it, there was so much to see and do and we think we made a small impact visiting for the day," she said.
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